Dab tsi yog cov txheej txheem sib xyaw ua ke siv rau kev sib cais thiab ua kom huv ntawm cov khoom fermentation?
1. Evaporation concentration:siv cov cua sov kom vaporise cov dej hauv cov kua fermentation, yog li ua kom cov concentration ntawm cov khoom.
Qhov zoo:Kev ua haujlwm yooj yim, tus nqi qis ntawm cov cuab yeej siv, muaj feem xyuam rau cov concentration ntawm ntau yam khoom fermentation, thiab tuaj yeem ua tau sai thiab ua tau zoo kom cov concentration ntawm cov khoom.
Qhov tsis zoo:Rau qee cov khoom lag luam kub-rhiab heev fermentation, kub siab tuaj yeem ua rau lawv cov denaturation thiab inactivation; Nyob rau tib lub sijhawm, cov txheej txheem evaporation yuav tsum tau siv ntau lub zog, tus nqi siab. Piv txwv li, evaporation concentration yuav siv tau thaum concentrating ib co amino acid fermentation broths uas tsis dhau rhiab heev rau kub, tab sis nws yuav tsum tau siv nrog ceev faj rau ib co protein fermentation khoom.
2. Membrane sib cais concentration:
Ultrafiltration:kev siv ultrafiltration membrane sieving nyhuv, cia me me molecules xws li dej, inorganic ntsev, thiab lwm yam los ntawm, thaum lub loj molecules ntawm cov khoom fermentation yog khaws cia nyob rau sab ntawm daim nyias nyias, thiaj li mus cuag concentration.
Qhov zoo:nws tuaj yeem ua haujlwm ntawm chav tsev kub, uas tsis tshua muaj kev cuam tshuam rau kev ua haujlwm ntawm cov khoom fermentation; nws tuaj yeem tshem tawm impurities hauv fermentation broth thiab txhim kho cov purity ntawm cov khoom; cov txheej txheem khiav lag luam yog yooj yim thiab yooj yim rau automate tswj.
Qhov tsis zoo:tus nqi ntawm daim nyias nyias yog siab thiab yooj yim rau cov kab mob, yuav tsum tau tu thiab tu; rau qee qhov impurities nrog qhov hnyav molecular zoo ib yam li cov khoom lag luam, nws yuav tsis tuaj yeem tshem tawm tag nrho.
Nanofiltration:Nws cov membrane pore loj yog nyob nruab nrab ntawm ultrafiltration membrane thiab thim rov qab osmosis membrane, nyob rau hauv siab, nws tso cai rau ib co me me molecules thiab monovalent ions hla, thaum nws muaj ntau dua retention tus nqi rau divalent thiab saum toj no ions thiab fermentation khoom nrog loj molecular hnyav, thiaj li. ua kom muaj concentration thiab sib cais.
Qhov zoo:Nws muaj cov nyhuv concentration zoo dua, tuaj yeem ua haujlwm nyob rau hauv qis qis thiab siv zog tsawg; nws muaj kev cuam tshuam tsawg dua rau cov khoom siv hluav taws xob kub thiab tuaj yeem tswj xyuas cov haujlwm ntawm cov khoom.
Qhov tsis zoo:qhov kev xaiv ntawm nanofiltration membrane yog qhov tsawg, qee qhov impurities yuav khaws cia ua ke nrog cov khoom lag luam; lub neej kev pab cuam ntawm daim nyias nyias yog txwv thiab yuav tsum tau hloov lub sij hawm.
3. Kev Cia Siab: Dawbze lub fermentation broth mus rau hauv qab lub freezing point, thiaj li hais tias cov dej nyob rau hauv nws cov ntaub ntawv ice crystals, thiab ces cais cov dej khov crystals los ntawm cov concentrated broth los ntawm centrifugation, pom, thiab lwm yam, thiaj li mus cuag lub hom phiaj ntawm concentration.
Qhov zoo:Nws yog qhov tsim nyog rau cov concentration ntawm cov khoom cua sov-rhiab heev fermentation vim tias cov haujlwm ntawm cov khoom tuaj yeem khaws cia rau qhov siab tshaj plaws los ntawm kev ua haujlwm ntawm qhov kub thiab txias; nws yuav tsis qhia lwm yam tshuaj lom neeg thiab yuav muaj kev cuam tshuam tsawg dua rau kev purity ntawm cov khoom.
Qhov tsis zoo:cov txheej txheem kev ua haujlwm yog qhov nyuaj dua, yuav tsum muaj cov khoom siv khov tshwj xeeb thiab cov khoom sib cais, nrog cov nqi ntau dua; Lub concentration multiplier yog qhov tsawg, thiab nws yuav xav tau ntau txoj haujlwm kom ua tiav qhov xav tau concentration.
4. nag lossis daus concentration:precipitate cov khoom fermentation los yog impurities los ntawm kev ntxiv precipitant, thiab ces cais cov precipitates, thiaj li mus cuag lub concentration ntawm fermentation broth. Piv txwv li, rau cov khoom fermentation protein, organic solvents, ntsev thiab lwm yam precipitants yuav muab ntxiv rau precipitate cov proteins, thiab ces tshem tawm cov supernatant kom ua tiav lub hom phiaj ntawm concentration.
Qhov zoo:kev ua haujlwm yooj yim, tus nqi qis; tuaj yeem ua tiav cov khoom lag luam thiab kev ua kom huv ua ntej tib lub sijhawm.
Qhov tsis zoo:kev xaiv ntawm precipitant yuav tsum tau ua raws li qhov xwm ntawm cov khoom fermentation, txwv tsis pub nws yuav cuam tshuam rau kev ua si thiab purity ntawm cov khoom; cov txheej txheem nag lossis daus tuaj yeem cuam tshuam los ntawm pH tus nqi ntawm cov tshuaj, qhov kub thiab txias thiab lwm yam, uas yuav tsum tau tswj nruj me ntsis.
5. Dialysis concentration:Siv cov nyhuv lim dej ntawm cov khoom siv semi-permeable, cov me me hauv cov tshuaj fermentation xws li dej, inorganic ntsev, thiab lwm yam yuav raug dialysed, thaum lub loj molecules ntawm cov khoom fermentation yuav nyob twj ywm nyob rau hauv lub dialysis hnab, thiaj li mus cuag. concentration.
Qhov zoo:Kev ua haujlwm yooj yim, tsis xav tau cov cuab yeej tshwj xeeb; tuaj yeem ua haujlwm ntawm chav tsev kub, tsis tshua muaj kev cuam tshuam rau kev ua haujlwm ntawm cov khoom fermentation.
Qhov tsis zoo:lub concentration ceev yog qeeb thiab nws yuav siv sij hawm ntev kom ncav cuag qhov xav tau concentration; tus nqi ntawm lub hnab lim ntshav yog siab thiab nws yooj yim tawg, yog li nws yuav tsum tau ua tib zoo saib xyuas.