Yuav ua li cas optimize cov txheej txheem extraction ntawm collagen los ntawm daim tawv nqaij tilapia?
Los ntawm ultrasonic assisted enzymatic hydrolysis, qhov tau collagen protein muaj siab yield thiab zoo txheeb ze solubility, thiab yog dav siv nyob rau hauv cov khoom noj.
Nyob rau hauv ib tug tej yam hwj chim ntau yam, cov nyhuv cavitation ua los ntawm ultrasound thiab
Cov teebmeem kev siv tshuab tuaj yeem hloov kho cov qauv ntawm cov enzyme, nthuav tawm nws qhov chaw nquag, thiab txhim kho cov haujlwm ntawm cov enzyme. Ultrasound kuj tseem tuaj yeem hloov cov khoom ntawm substrate, ua kom yooj yim rau collagen kom yaj hauv cov kuab tshuaj extraction, muab cov xwm txheej zoo tshaj plaws rau collagen extraction.
Khoom qauv
Ultrasonic pab extraction yog ib txoj kev tshiab extraction nyob rau hauv xyoo tas los no. Lub mechanism ntawm nws pab extraction ntawm collagen yog siv lub siab loj generated los ntawm ultrasonic cavitation kom lub uncrosslinked collagen molecules sib cais instantaneously.
Cov teebmeem ntawm cov khoom siv tom qab ultrasound pab txhawb kev sib cais thiab nce tus nqi ntawm collagen rho tawm, thaum kho ultrasound qhib cov collagen fibers los pab txhawb kev kho cov kua qaub thiab enzyme. Piv nrog rau cov txheej txheem ib txwm siv, lub sijhawm rho tawm tuaj yeem ua kom luv luv.
Nyob rau hauv cov txheej txheem ntawm ultrasonic-pab extraction, cov ntsiab lus ntawm hom I collagen nyob rau hauv raw cov ntaub ntawv, ultrasonic lub sij hawm, thiab ultrasonic ib chav tsev yuav cuam tshuam tus nqi extraction. Yog li ntawd, tsawg zaus high-power ultrasound feem ntau yog siv nyob rau hauv kev lag luam los pab cov extraction ntawm collagen.
Kev khiav dej num
Siv ib co nyiaj ntawm cov saum toj no kho ntses nplai hmoov, ntxiv ib tug npaum li cas ntawm acetic acid tov thiab enzymes (raws li yuav tsum tau), muab tso rau hauv lub ultrasonic cell crusher extraction. Tom qab qhov kawg ntawm ultrasound, lub raj mis conical tau muab tso rau ntawm qhov ntsuas kub kom haum rau cov tshuaj tiv thaiv, thiab tag nrho lub sijhawm raug tswj tsis pub dhau 8 teev. Tom qab lub enzymatic hydrolysis, centrifuge ntawm 8000 r / min rau 20 feeb, thiab khaws cov supernatant.
