1. Cov txiaj ntsig siab dua
Kev txhim kho kev ua haujlwm zoo yog qhov laj thawj tseem ceeb rau kev hloov pauv los ntawm kev tuav lub fwj mus rau bioreactors, qhov uas koj tuaj yeem tau txais ntau dua biomass lossis cov khoom tsim los ntawm cov hlwb hauv lub sijhawm luv. Piv txwv li, cov txiaj ntsig ntawm kev cog qoob loo K12 Escherichia coli nyob rau hauv co fwj thiab bioreactors yog qhov sib txawv rau cov tsiaj qus.
2. Cov ntaub ntawv ntawm cov txheej txheem lom neeg lub sijhawm
Vim lub fact tias bioreactors cia siab rau microcomputers ncaj qha tswj ntau yam parameter cov ntaub ntawv, thiab muaj peev xwm ua tau raws li lub sij hawm ntawm kev sib kis ntawm plasma cell loj hlob raws li txoj cai, yaj tsis muaj oxygen, DO qhov tseem ceeb, conductivity, thiab lwm yam los ntawm ntau yam sensors, real-time txheej txheem tswj tsis tau. yuav tsum tau ua tiav thaum lub sij hawm shake flask cultivation. Cov hauv qab no yog kev sib piv nrog cov qauv cog qoob loo hauv bioreactors:
Txawm hais tias tuav lub raj mis nrog cov khoom siv thib peb tuaj yeem ua tau ntau dua, cov nqi, kev tuav, thiab kev sib cuam tshuam cov teeb meem txhua tus ua rau muaj kev daws teeb meem rau cov kev siv tshwj xeeb thiab ib puag ncig.
3. Adaptability
Ib qho kev sib txawv loj ntawm co fwj thiab bioreactors yog tias kev siv bioreactors txhim kho kev hloov pauv. Qhov no tsis yog hais txog tus naj npawb ntawm tus neeg tsis muaj peev xwm ntsuas tau thiab tswj tau. Qhov zoo thiab ntau yam ntawm kev ntsuas qhov tseem ceeb, nrog rau kev muaj peev xwm tswj tau lawv, feem ntau txwv ntau dua hauv cov fwj tuav. Feem ntau, kev tswj hwm qhov tseem ceeb xws li qhov kub thiab txias yog siv ib txhij rau txhua lub fwj tuav. Rau ntau daim ntawv thov lim, qhov no yuav tsis yog qhov tseem ceeb, tab sis nws yog ib qho teeb meem rau kev tshawb fawb optimization.
4. Kev tswj kev lom neeg zoo dua
Qhov no yog qhov tseem ceeb ntawm tus nqi ntawm kev hloov pauv ntawm cov fwj tuav mus rau bioreactors. Qhov no tuaj yeem ua tiav los ntawm kev siv cov tswv yim phased muab los ntawm cov txheej txheem lom neeg siv los tswj tus kheej tsis tau lossis ua tiav cov txheej txheem tswj. Kev tuav lub raj mis tuaj yeem ua tiav qee cov tswv yim tswj kev muaj txiaj ntsig, tab sis lawv yog txwv tsis pub siv los yog xav tau cov khoom siv ntxiv.
5. Nthuav cov muaj peev xwm
Vim muaj qhov nce ntxiv hauv kev tsim khoom ntau xyoo dhau los, ib qho me me ntawm kab lis kev cai tuaj yeem muaj cov xov tooj ntawm tes siab lossis cov khoom lag luam concentration. Qhov no ua rau co lub raj mis kab lis kev cai yog qhov ua tau rau kev tsim khoom me me hauv kev siv thaum ntxov. Txawm li cas los xij, nyob rau hauv qhov xav tau nthuav dav mus rau theem kev lag luam, me me bioreactors tuaj yeem tsim cov qauv raug thiab muab lub hauv paus ruaj khov. Qhov no yog vim qhov yooj yim lub cev zoo sib xws thiab zoo ntawm cov ntaub ntawv muaj, xws li:
Stirring stage reactors (STR's) tseem yog ib hom kev tsim cov nplai bioreactors, thiab chav kuaj ntsuas bioreactors tuaj yeem ua tau zoo dua qhov no. Piv txwv li, cov qauv kev nthuav dav tseem ceeb xws li impeller tip ceev thiab ntim loj hloov pauv muaj feem xyuam rau bioreactors, tab sis tsis ncaj qha cuam tshuam nrog cov khoom sib txuas. Kev tswj cov tswv yim thiab kev ua kom zoo ntawm cov bioreactors me me feem ntau tuaj yeem siv rau ntawm qhov loj dua los ntawm kev hloov pauv yooj yim.
Hauv cov ntsiab lus, txawm tias bioreactors qee zaum suav tias yog qhov nyuaj, kim, thiab siv sijhawm. Tab sis nyob rau hauv bioreactors, ntau yam ntsuas tau raug tswj kom raug, thiab txawm tias EKF lactate analyzer tuaj yeem siv rau kev tshawb xyuas lub sijhawm tiag tiag thiab tshawb pom cov lactate tsim tawm thaum lub sijhawm cell kab lis kev cai kom ntseeg tau tias qhov chaw loj hlob ntawm tes zoo.
Yog tias koj xav ua kom cov qoob loo ntau ntxiv, nthuav cov nplai ntawm cov kab lis kev cai ntawm tes, thiab ntsuas cov txheej txheem, siv lub desktop bioreactor yuav yog koj qhov kev xaiv raug.