Muab rho tawm -} carotene los ntawm microalgae yog qhov kev txhim kho tseem ceeb rau cov xim muaj xim thiab cov khoom xyaw ua haujlwm.
Supercritical koom kev rho tawm
Lub ntsiab lus
Siv cov kev ua kom siab thiab solubility ntawm co₂ nyob rau hauv supercritical cov kev mob (kev kub siab thiab kub} carotene.
Kev ua
- Pretreatment:Qhuav thiab pulverize microalgae (xws li dunaliella salina) kom cuam tshuam nrog phab ntsa ntawm tes.
- Extraction tej yam:
Siab: 30-40 MPa (30 MPa rau Dunaliella Salina, 40 MPA rau lwm cov microalgae)
Qhov kub: 45-60℃(45℃rau dunaliella salina; 60℃rau scenedesescus almeriensis)
CO₂ YOOJ YIM: 10 L / H (Dunaliella Salina) lossis 1 G / Min (Rau Lwm Algae)
Sijhawm: 5-6 teev.
- Txhim Kho:Ntxiv tus EntRainer (xws li Ethanol) kom nce cov solubility ntawm - carotene hauv CO₂.
Qhov Zoo
- Extraction cov txiaj ntsig nce mus txog li 95.09% (salina), thiab lub CIS {{} om om om om om om om om om om om om om om om om om om is,, reach reach reach reach reach............ Reach.
- Tsis muaj cov kuab tshuaj seem, muaj cov khoom lag luam siab, thiab ua raws li cov qauv khoom noj / chaw muag tshuaj.
Aqueous ob -} theem ntu extraction (ATPS)
Lub ntsiab lus
Ib lub zog hydrothilic (dej cawv) yog siv los ua ob -}} {{{{} carotene hauv cov organic theem.
Cov Tshuab thiab Cov Txheej Txheem
- Ethanol - acetone / ammonium sulfate system (rau spirulina):
15% (etethanol + acetone) + 24% ammonium sulfate;
6% cov khoom -} kua piv, pH 8.0, extraction ntawm 30 degree, extraction paib 94.55%.
- Ethangol - n - butanol system:
8.2% ethanol + 36} n {2} {} {} {, our {, tawm ntawm 3 feeb, tawm los 3.13 mg / g.
- Tert - butanol - maktose system:
Ultrasound {- pab extraction extraction (90 w, 5 min), tawm los 3.19 mg / g.
Ultrasound -} pab tau cov kuab tshuaj thaiv kev extraction
Lub ntsiab lus
Ultrasonic cavitation rhuav tshem lub microalgae cell phab ntsa, ua kom muaj kev nkag siab ntau.
Cov lus qhia ua haujlwm tseem ceeb
- Hnyav ua ke:
Roj av ether {{- acetone, methanol {{} acetone, lossis tetrahydrofuran;
n - buthanol {{}}} ethanol sib tov (txo kev siv cov kuab tshuaj lom).
- Parameter Optimization:
Ultrasound Power: 90-325 W;
Lub Sij Hawm: 3-5 feeb (ntau dhau kev siv yuav ua rau muaj kev sib cav).
Enzyme - pab tau extraction
Lub ntsiab lus
Cellulase thiab pectinase hydrolyze lub microalgae cell phab ntsa kom tso cov txheej txheem intracellular.
Kev ua
- Microalgae slurry yog pretreated nrog kev daws enzyme (pH 4.5-5.0) ntawm 40-50 degree;
- Cov kev rho tawm theem nrab theem nrab yog ua nrog cov organic huab cua (piv txwv li, n- hexane).
Kev txwv
Cov enzymes yooj yim inactivated hauv cov kuab tshuaj organic, yog li lub sij hawm ntawm cov kuab tshuaj ntxiv yuav tsum tau ua tib zoo tswj tau.
Microwave - pab tau extraction
Cov kauj ruam
- Microalgae hmoov yog sib xyaw nrog 95% ethanol thiab acetone (piv txwv li, 1: 2 piv);
- Microwave kev kho yog ua (hwj huam 300-600 w) rau tsawg dua 10 feeb.
Kev pheej hmoo
Kev kub siab heev yuav ua rau thermal degradation lossis isomerization ntawm -} carotene.
Tshuab sib piv thiab cov lus pom zoo
| Txojkev | Extraction tus nqi / tawm los | Sij hawm | Muaj feem tau microalgae hom | Muaj Peev Xwm |
| Supercritical koom kev rho tawm | 95.09% | 5-6 teev | Dunaliella, Spirulina | (Cov khoom siv muaj nqi siab) |
| Aqueous ob -} theem ib qho | 94.55% | <30 minutes | Tus kauv | ★★★★ |
| Ultrasound -} pab tau cov kuab tshuaj thaiv kev extraction | >90% | 3-5 teev | Ntau yam microalgae | ★★★★ |
| Enzymatic Hydrolysis | 80–85% | 1-2 teev | Microalgae nrog Thicker Cell Phab Ntsa | ★★★ |
Cov kev txiav txim siab tseem ceeb
- Kev tswj ruaj khov: {{{- carotene yog yooj yim degraded los ntawm lub teeb, kub, thiab oxygen. Tag nrho cov txheej txheem yuav tsum tau ua nyob rau hauv qhov tsaus ntuj thiab hauv qab nitrogen.
- Kev siv cov khoom seem Algal: piv txwv li, cov seem ntawm {}}}} pa ntxiv rau anti- Cov kauj ruam cov neeg tiv thaiv lub zog.
- Cov kuab tshuaj nyob ntawm cov khoom noj / kev siv zaub mov / kws kho mob, nws yuav tsum ua raws li GB 2760 lossis supercritical, thiab qis}} paim aqueous system yog nyiam.