Daim Ntawv Thov ntawm Bioreactor hauv Monoclonal Antibody ntau lawm

Cov tshuaj tiv thaiv monoclonal yog cov tshuaj tiv thaiv homogeneous tsim los ntawm ib leeg B-Cell clone, qhia tawm tsam ib qho kev tawm tsam antigenic. Lawv npaj kev vam meej ntawm Hybridoma Technology: Kev txhaj tshuaj Cov kev tawm tsam no yog tshwj xeeb heev thiab tuaj yeem paub meej txog cov hom phiaj xws li cov kab mob cov kab mob xws li cov cim qog, thiab siv dav hauv cov tshuaj thiab kev tshawb fawb. Lawv tau siv rau kev kho mob cancer (piv txwv li, kab mob autoimmune) los ntawm kev thaiv cov kab mob (piv txwv li cov cim neocoronazole) rau lub cev tiv thaiv kab mob kom tshem tawm. Tsis tas li ntawd, lawv kuj tseem siv hauv kev kuaj mob (piv txwv li ELISA ua haujlwm) thiab cov protein hauv zos kev soj ntsuam hauv kev tshawb fawb txog roj ntsha hauv kev tshawb fawb txog kev muaj roj ntsha. Piv rau cov tshuaj tiv thaiv polyclonal, cov tshuaj tiv thaiv monoclonal muaj cov high batch stability thiab tsawg dua crossity, tab sis muaj nqi ntau dua los txhim kho. Nyob rau hauv xyoo tas los, tib neeg cov tshuaj tiv thaiv hloov los ntawm cov caj ces engineering tau txo qis kev pheej hmoo ntawm kev tiv thaiv tsis muaj zog, ntxiv txhawb nqa lawv cov tshuaj kho.

Bioractor yog cov khoom siv tseem ceeb ntawm kev tsim cov tshuaj tiv thaiv monoclonal ntawm cov tshuaj tiv thaiv kab mob hlwb (piv txwv li xov tooj ntawm hlwb) los ntawm simulating ntawm tes loj hlob ib puag ncig.

Kev nthuav dav ntawm tes ntawm tes thiab hais tawm

• Batch \/ txhawb kev cai lij choj: Tswj cov kev ua haujlwm cell metabolic, txuas ntxiv ntawm kev sib koom ua ke (37 degree), pH (10% {{{{{} {%).
• Ncau kab lis kev cai: Combined with cell retention technology (hollow fiber membrane or centrifugation), continuous replenishment of fresh medium and removal of metabolic wastes (e.g. lactic acid, ammonia), the cell density can reach >1 × 10 ^ 7 hlwb \/ ml, uas txhim kho cov tshuaj tiv thaiv monoclonal tawm.

Cov Txheej Txheem Tswj thiab Txhim Kho

• Parameter Online soj xyuas: Kev tswj lub sijhawm tiag tiag los ntawm kev ua, pH, qab zib, lactate fluxolic kev tsom xam, ua ke nrog kev ua kom zoo dua cov qauv ntawm cov khoom lag luam.
• Kev Sib Tw Hlau: Txuas nrog Pat (Txheej Txheem Ntsuam Xyuas Tshuab) kom kho tau RPM),}} rpm),} ripation thiab batch-to-batch linency (CV<5%).

Yooj thiab hloov kho

• Reactor hom: Los ntawm kev sim (1-10 lirrred qib ({} l stainless hlau \/} l

Kev Tswj Xyuas Zoo

• Tshuaj tiv thaiv kev ruaj khov ruaj khov: Txo cov protein ntau yam lossis tsis zoo los ntawm cov tshuaj tiv thaiv qis (piv txwv li qhov kub thiab txias antibodys)

Kev sib xyaw ua ke ntawm cov thev naus laus zis

• Cov txheej txheem ntau lawm: Tandem kev sib txuas ntawm ntau tus bietactors kom paub cov cell loj dua thiab nce ntau lawm ntau lawm tau los ntawm {1}} lub sij hawm.
• Gene Editing xov tooj yoog: Txhim kho cov tswv yim Reactor pub zaub mov (piv txwv li cov hnab looj tes zoo nkauj

Cov piv txwv thov

• Seccical Qib Ntau Lawm: Using 200 L disposable bioreactor, a single batch can produce >1 kg ntawm monoclonal tshuaj tiv thaiv kom tau raws li kev xav tau ntawm pd -1 \/ pd-l1 tshuaj tua kab tshuaj.
• Kev Kho Mob Tus Kheej: Micro Bioreactor (Ambr System) rau kev soj ntsuam sai ntawm tus neeg mob-cov txheej txheem tshwj xeeb tiv thaiv kev txwv.

Kev thev naus laus zis ua haujlwm hauv Biorkactors (piv txwv li cov kev cai lij choj uas tsav tsheb loj, txo cov tshuaj tiv thaiv monoclonal